u87mg cell lines  (ATCC)

Journal: International Journal of Nanomedicine

Article Title: Transferrin-Functionalized Conjugated Polymer Nanoparticles for Enhanced Photodynamic Therapy of Glioblastoma

doi: 10.2147/IJN.S592688

Figure Lengend Snippet: Integrated in silico and experimental analysis of transferrin receptor 1 (TfR1 / TFRC) expression in gliomas and representative cell lines. Box-plot summary of TFRC mRNA expression obtained from GEPIA (Gene Expression Profiling Interactive Analysis) based on tumor and normal samples from the TCGA and the GTEx databases (accessed March 2025). ( A ) Comparison of TFRC expression in high-grade gliomas (GBM) vs. low-grade glioma (LGG) (n indicated on each plot). ( B ) TFRC expression stratified by canonical GBM molecular subtype (classical, mesenchymal, neural, proneural). Boxes represent the interquartile range, horizontal lines the median, whiskers extend to 1.5×IQR, and individual data points are overlaid. Brackets with asterisks denote statistically significant pairwise differences (see Methods for statistical test). ( C ) TfR1 expression levels in GBM cell lines and HEK293 (non-tumor control). Data taken and adapted from the Human Protein Atlas. ( D ) Representative flow-cytometry histograms of surface TfR1 staining in U87MG, T98G, MO59K and HEK293 cells. Traces correspond to unstained control (red), secondary-only control (anti-mouse IgG–AF647; Orange) and specific anti-CD71 primary staining followed by anti-mouse-AF647 secondary (blue). The horizontal bracket on each histogram indicates the gate used to define TfR1-positive events. ( E ) gMFI of the CD71 signal (mean ± SD; n = biological replicates indicated in Methods), and ( F ) percentage of TfR1-positive cells. Data were normalized to appropriate controls. Statistical comparisons were performed as described in Methods (* p < 0.05 ).

Article Snippet: Moreover, analysis of mRNA expression data from the Human Protein Atlas indicates that the U87MG cell line (derived from GBM) exhibits elevated TFRC/TfR1 expression, whereas HEK293 cells (of human embryonic kidney origin) display minimal receptor expression ( ).

Techniques: In Silico, Expressing, Gene Expression, Comparison, Control, Flow Cytometry, Staining

Journal: International Journal of Nanomedicine

Article Title: Transferrin-Functionalized Conjugated Polymer Nanoparticles for Enhanced Photodynamic Therapy of Glioblastoma

doi: 10.2147/IJN.S592688

Figure Lengend Snippet: Time-course and competition analysis of cellular uptake of F8BT CPNs with and without holo-Tf. ( A and B ) Representative flow-cytometry histograms showing nanoparticle-associated fluorescence in U87MG and T98G cells at the indicated incubation times (0.5, 1, 2 and 4 h). Unexposed control cells (Ctrl) are shown in red; histograms corresponding to successive time points are overlaid to illustrate the progressive accumulation of nanoparticle signal. ( C and D ) Quantification of uptake kinetics derived from flow-cytometry data, expressed as geometric mean fluorescence intensity (gMFI) as a function of incubation time for non-functionalized F8BT CPNs and holo-Tf-functionalized F8BT-Tf CPNs. Error bars represent SD. ( E ) Competition assay performed 2 h post-incubation showing that the presence of excess free holo-Tf significantly reduces the uptake of F8BT-Tf CPNs relative to non-competed F8BT-Tf CPNs, whereas uptake of non-functionalized F8BT CPNs is minimally affected. ( F ) Representative confocal micrographs showing intracellular localization of CPNs (green), F-actin (rhodamine-phalloidin, red), and nuclei (Hoechst, blue) after 30 min exposure. Orthogonal (xz) projections confirm intracellular, sub-membrane localization of the punctate nanoparticle signal; dashed lines indicate the focal plane used for orthogonal projections. Scale bars = 20 µm. Statistical significance: ns, not significant; * p < 0.05 ; ** p < 0.01 ; *** p < 0.001 ; **** p < 0.0001 .

Article Snippet: Moreover, analysis of mRNA expression data from the Human Protein Atlas indicates that the U87MG cell line (derived from GBM) exhibits elevated TFRC/TfR1 expression, whereas HEK293 cells (of human embryonic kidney origin) display minimal receptor expression ( ).

Techniques: Flow Cytometry, Fluorescence, Incubation, Control, Derivative Assay, Competitive Binding Assay, Membrane

Journal: International Journal of Nanomedicine

Article Title: Transferrin-Functionalized Conjugated Polymer Nanoparticles for Enhanced Photodynamic Therapy of Glioblastoma

doi: 10.2147/IJN.S592688

Figure Lengend Snippet: Photodynamic cytotoxicity of F8BT-PtOEP CPNs with and without holo-Tf in GBM cell lines. ( A ) Cell viability of U87MG and ( B ) T98G cells after 24 h incubation with increasing concentrations (1–10 mg·L −1 ) of F8BT-PtOEP CPNs or F8BT-PtOEP-Tf CPNs, followed by blue light irradiation (40 J·cm −2 ). Cell viability was determined 24 h post-irradiation using the MTT assay. Data are expressed as mean ± SD (n = 6). Curves represent independent nonlinear regression fits generated in GraphPad Prism for each treatment group. Asterisks indicate statistically significant differences between treatments (two-way ANOVA, * ***p < 0.0001 ). ( C ) Representative phase-contrast and fluorescence microscopy images (20×) showing intracellular ROS production immediately after PDT, as measured by DCFDA staining (green fluorescence). In both U87MG and T98G cells, increased green fluorescence intensity correlates with holo-Tf functionalization and increased CPNs internalization, indicating enhanced ROS generation. Control (light only) samples show negligible fluorescence, confirming CPNs and light-dependent activation. Scale bar = 100 µm.

Article Snippet: Moreover, analysis of mRNA expression data from the Human Protein Atlas indicates that the U87MG cell line (derived from GBM) exhibits elevated TFRC/TfR1 expression, whereas HEK293 cells (of human embryonic kidney origin) display minimal receptor expression ( ).

Techniques: Incubation, Irradiation, MTT Assay, Generated, Fluorescence, Microscopy, Staining, Control, Activation Assay

Journal: International Journal of Nanomedicine

Article Title: Transferrin-Functionalized Conjugated Polymer Nanoparticles for Enhanced Photodynamic Therapy of Glioblastoma

doi: 10.2147/IJN.S592688

Figure Lengend Snippet: In vivo therapeutic evaluation, biodistribution, and experimental workflow of F8BT-PtOEP CPNs with and without holo-Tf in an orthotopic U87MG-tdiRFP GBM model. ( A ) Schematic overview of the experimental design. U87MG-tdiRFP cells were stereotactically implanted on day 0. Nanoparticle formulations (1 mg·kg −1 , i.v.) were administered on day 10, and PDT was applied 24 h later (day 11). Illumination was delivered through a minimal cranial trepanation using a fiber-optic LED source (irradiance 20 mW·cm −2 at the fiber tip, 10 min; total fluence 12 J·cm −2 ) and was delivered through a minimal cranial trepanation created at the tumor implantation site to allow direct transmission of light into the brain parenchyma while minimizing attenuation by the skull. Animals were subsequently monitored for survival. ( B ) Biodistribution of Pt-containing CPNs 24 h after systemic administration in tumor-bearing mice (n = 4 per group). Platinum levels were quantified by ICP-MS. Both formulations showed predominant hepatic accumulation, consistent with reticuloendothelial clearance of polymeric nanoparticles. Detectable Pt levels in brain tissue were observed only in the F8BT-PtOEP-Tf CPNs group, whereas Pt concentrations in mice receiving F8BT-PtOEP CPNs remained below the analytical limit of detection (LOD = 1 ppb). ( C ) Representative ex vivo NIR fluorescence images of excised brains collected at humane endpoint. Tumor burden derived from the tdiRFP reporter is shown in pseudocolor (red) over a grayscale anatomical background. Images were acquired under identical imaging conditions and correspond to the following experimental groups (displayed uniformly across panels): Control, Light only, F8BT-PtOEP CPNs, F8BT-PtOEP CPNs + Light, F8BT-PtOEP-Tf CPNs, and F8BT-PtOEP-Tf CPNs + Light. Fluorescence intensity scale is shown below. Scale bar = 5 mm. ( D ) Quantification of ex vivo tumor fluorescence area (mean ± SD, n = 4 per group), demonstrating the greatest reduction in tumor signal in animals treated with F8BT-PtOEP-Tf CPNs followed by light irradiation. ( E ) Kaplan–Meier survival analysis for all treatment groups (n = 6 per group). The F8BT-PtOEP-Tf CPNs + Light group showed the most pronounced survival benefit, followed by the F8BT-PtOEP CPNs + Light group, whereas nanoparticle-only and light-only controls produced no significant improvement. Statistical significance was evaluated using the log-rank (Mantel–Cox) test. ** p < 0.01 , **** p < 0.0001 . Illustrations created with BioRender.

Article Snippet: Moreover, analysis of mRNA expression data from the Human Protein Atlas indicates that the U87MG cell line (derived from GBM) exhibits elevated TFRC/TfR1 expression, whereas HEK293 cells (of human embryonic kidney origin) display minimal receptor expression ( ).

Techniques: In Vivo, Tumor Implantation, Transmission Assay, Ex Vivo, Fluorescence, Derivative Assay, Imaging, Control, Irradiation, Produced

Journal: International Journal of Nanomedicine

Article Title: Transferrin-Functionalized Conjugated Polymer Nanoparticles for Enhanced Photodynamic Therapy of Glioblastoma

doi: 10.2147/IJN.S592688

Figure Lengend Snippet: Histopathological evaluation of orthotopic U87MG-tdiRFP GBM following CPNs treatment and PDT. Representative hematoxylin–eosin–stained brain sections from each experimental group (n = 4) are shown, highlighting key morphological features associated with treatment response. Control tumors exhibit compact, highly cellular GBM architecture with moderate–marked pleomorphism and abundant blood vessels (black arrowheads: mitotic figures). Light-only controls display diffuse infiltration into adjacent parenchyma and neoformed aberrant vasculature (black arrow: perivascular neoplastic cells infiltrating into the brain parenchyma. The absence of a boundary with normal tissue is noted). In the non-irradiated F8BT-PtOEP-Tf CPNs -treated group, regions of partially preserved parenchyma are interspersed with infiltrating tumor cells (white arrows). GBM treated with non-functionalized F8BT-PtOEP CPNs + PDT shows extensive coagulative necrosis and hemorrhage, irregular hyperemic vessels, and palisading tumor-cell arrangements. The F8BT-PtOEP-Tf CPNs + PDT group demonstrates the most severe tissue disruption, with widespread necrosis, hemorrhage, and marked vascular damage, together with reduced mitotic activity and abundant cell death (yellow arrowhead: pyknosis). Scale bars = 100 (10x magnification) and 50 μm (40x magnification). The non-irradiated F8BT-PtOEP CPNs group is not shown for clarity, as its histological features were comparable to those observed in the other nanoparticle-treated group under dark conditions.

Article Snippet: Moreover, analysis of mRNA expression data from the Human Protein Atlas indicates that the U87MG cell line (derived from GBM) exhibits elevated TFRC/TfR1 expression, whereas HEK293 cells (of human embryonic kidney origin) display minimal receptor expression ( ).

Techniques: Staining, Control, Irradiation, Disruption, Activity Assay

Journal: Frontiers in Pharmacology

Article Title: AI-driven network pharmacology and multi-omics validation identify KCNH2 as a prognostic biomarker and candidate therapeutic vulnerability of Acorus tatarinowii in glioblastoma

doi: 10.3389/fphar.2026.1811032

Figure Lengend Snippet: Differential expression of KCNH2 and validation of stable knockdown efficiency in glioblastoma cells. (A) Relative mRNA expression of KCNH2 in NHA, U87MG, and LN229 cells measured by quantitative PCR. (B,C) Relative KCNH2 mRNA levels in U87MG and LN229 cells transduced with shNC or three independent shRNAs targeting KCNH2. Data are presented as mean ± SD from three independent experiments.

Article Snippet: The human glioblastoma cell lines U87MG (RRID: CVCL_0022) and LN229 (RRID: CVCL_0393), as well as the human embryonic kidney cell line HEK293T (RRID: CVCL_0063), were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Quantitative Proteomics, Biomarker Discovery, Knockdown, Expressing, Real-time Polymerase Chain Reaction, Transduction

Journal: Frontiers in Pharmacology

Article Title: AI-driven network pharmacology and multi-omics validation identify KCNH2 as a prognostic biomarker and candidate therapeutic vulnerability of Acorus tatarinowii in glioblastoma

doi: 10.3389/fphar.2026.1811032

Figure Lengend Snippet: KCNH2 knockdown impairs clonogenicity, proliferation, and migration in glioblastoma cells. (A–C) Representative colony formation images and quantitative analysis of colony numbers in U87MG and LN229 stable cell lines transduced with shNC or three independent shRNAs targeting KCNH2. (D,E) CCK-8 growth curves of U87MG and LN229 cells following KCNH2 silencing, showing reduced cell viability over time. (F–I) Representative wound-healing images at 0 h and 48 h and quantitative analysis of migration rates in U87MG and LN229 cells. Data are presented as mean ± SD (n = 3).

Article Snippet: The human glioblastoma cell lines U87MG (RRID: CVCL_0022) and LN229 (RRID: CVCL_0393), as well as the human embryonic kidney cell line HEK293T (RRID: CVCL_0063), were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, Migration, Stable Transfection, Transduction, CCK-8 Assay

Journal: Frontiers in Pharmacology

Article Title: AI-driven network pharmacology and multi-omics validation identify KCNH2 as a prognostic biomarker and candidate therapeutic vulnerability of Acorus tatarinowii in glioblastoma

doi: 10.3389/fphar.2026.1811032

Figure Lengend Snippet: KCNH2 silencing triggers mitochondria-dependent apoptosis in glioblastoma cells. (A,C) Representative Annexin V-FITC/PI flow cytometry plots in U87MG (A) and LN229 (C) cells transfected with siNC or three independent siRNAs targeting KCNH2 (siKCNH2-1/2/3). (B,D) Quantification of total apoptotic cells (early + late apoptosis) in U87MG (B) and LN229 (D) . (E,F) Relative mRNA expression levels of mitochondrial apoptosis-related genes (BAX, BCL2, BCL2L1) in U87MG (E) and LN229 (F) ; transcript levels were normalized to GAPDH. (G,H) Mitochondrial membrane potential (ΔΨm) assessed by TMRM staining in U87MG (G) and LN229 (H) ; fluorescence intensity was normalized to the control group. (I,J) Cleaved caspase-3 levels measured by ELISA in U87MG (I) and LN229 (J) . Data are presented as mean ± SD (n = 3). Statistical significance is indicated in the figure.

Article Snippet: The human glioblastoma cell lines U87MG (RRID: CVCL_0022) and LN229 (RRID: CVCL_0393), as well as the human embryonic kidney cell line HEK293T (RRID: CVCL_0063), were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry, Transfection, Expressing, Membrane, Staining, Fluorescence, Control, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Pharmacology

Article Title: AI-driven network pharmacology and multi-omics validation identify KCNH2 as a prognostic biomarker and candidate therapeutic vulnerability of Acorus tatarinowii in glioblastoma

doi: 10.3389/fphar.2026.1811032

Figure Lengend Snippet: KCNH2 knockdown suppresses glioblastoma tumor growth in vivo . (A) Representative images of xenograft tumors excised from nude mice injected with shNC or shKCNH2 U87MG cells. (B) Tumor growth curves showing tumor volume progression over time. (C) Final tumor weights at the experimental endpoint. Data are presented as mean ± SD (n = 6). ***P < 0.001 versus shNC.

Article Snippet: The human glioblastoma cell lines U87MG (RRID: CVCL_0022) and LN229 (RRID: CVCL_0393), as well as the human embryonic kidney cell line HEK293T (RRID: CVCL_0063), were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Knockdown, In Vivo, Injection